Pseudomonas metabolically excreted end products, such as

Pseudomonas is a genus
of Gram-negative,
Gammaproteobacteria, belonging to the family Pseudomonadaceae
and containing 191 validly described species. The members of the genus
demonstrate a great deal of metabolic diversity and consequently are able to colonize
a wide range of niches. Infectious species include P. aeruginosa, P. oryzihabitans, and P. plecoglossicida. P.
aeruginosa flourishes in hospital environments, and is a particular problem
in this environment, since it is the second-most common infection in
hospitalized patients (nosocomial infections). This pathogenesis may
in part be due to the proteins secreted by P. aeruginosa. P. syringae
is a prolific plant pathogen. It exists as over 50 different pathovars,
many of which demonstrate a high degree of host-plant specificity. Numerous
other Pseudomonas species can act as plant pathogens, notably all of the
other members of the P. syringae subgroup, but P. syringae is the
most widespread and best-studied. Thus detection of the pseudomonas has become
very important in many fields of our day to day lives.

The detection of these organisms can easily
be done with the help of spectrometric detection. Here the first step is to
form a core of Ferric-Manganese oxide which is coated with silica. Iron (2) and
manganese may represent the primary electron acceptors for organic matter
oxidation in sedimentary environments where they are enriched. Bacterially
mediated Mn reduction can occur indirectly when reduced, metabolically excreted
end products, such as sulfide (3) and certain organic compounds (4, 5), react
abiotically with manganese oxides. Another important feature of the iron
manganese oxide is its magnetic properties. Silica-coated noble metal
nanoparticles have attracted great interest because they can be used as
catalysts as well as in calorimetric and optical applications. They are
typically produced using silane precursors, but these are generally insoluble
in water. Consequently, alcohol has to be added to water to facilitate the
hydrolysis of these precursors, increasing the cost of production and making
the process less green. The core is made to connect with the aptamer of a
specific sequence which is also complementary to the pseudomonas. The next step
is to add the sample containing pseudomonas. As a result it is expected that
all the pseudomonas are going to get attached to their complementary sequences
and the rest of the unwanted particles will be washed off.

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The last and the main part of the project
is the part of detection. This is done by connecting another aptamer to the
attached pseudomonas. These aptamers will already be connected to the ferric
manganese oxide cores. The main aim of this part is to send a signal on
attachment to the pseudomonas which can easily be specified either by
colorimetric or by mass spectrometric assay. The horseradish peroxide (HRP)
will be attached to the core. In the presence of peroxide when the 2, 4,
6-tribromophenol will be oxidized and a change in colour will be noticed. This
change in colour will only be noticed when the aptamer is attached to the
pseudomonas. Thus by doing spectrometric assay the optical density of the
solution can be obtained and by obtaining the change in the density the
concentration of the pseudomonas can easily be obtained.